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Flytraps acclimating from tissue culture

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Flytraps acclimating from tissue culture

Postby luxiloid » Sat Feb 04, 2012 6:18 am

Hi forum members,
I purchased some tissue cultures from FTS International and wanted to show you how the plants are acclimating.
I am growing my plants indoors using plant-LED lamps. In winter, my room is about 24 degrees Celcius and 40% in humidity. I live in South Korea and the coldest day in last week was -17 degrees Celcius (=1.4 degrees Fahrenheit).

These are the plants when they first arrived to me on 9th january.

IN_TC_01_resize.jpg
In tissue culture 01
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In tissue culture 02
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The Big Mouth was in a flask and they were so many! :D
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Big Mouth TC
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Here is the Big Mouth again after about 15 days. This plant does not look good right now because I put a plastic bag on it after two days of potting when I received them. Many leaves were lost due to low humidity for that two days.
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Big Mouth
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The rest of the plants look better because I put plastic bag on them to increase humidity without any delay thanks to Matt.
Here is Green Dragon. For each growing points, there are 3~4 new leaves since I potted them. The new leaves are growing so red that I cannot compare it to Red Dragon (Akai Ryu). I was expecting some greener margin or so but till now they are so identical. However, I am happy they are growing faster than typical red plants.
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Green Dragon
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Here is the famous B52. It had larger traps compared to the Big Mouth and Green Dragon even when it was inside the tube.
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B52
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Here is the A2 which is little brother of B52. Actually these were really growing too well in few days that I had repotted to divide them into different pots again. Can you believe that a plant just out of TC repotted two times in four weeks look like this? I have never seen a flytrap so vigorous like this one. some growing points already put out up to 5 new traps.
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A2
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The next plant is another amazing one called DC XL. It is as vigorous as the A2 and I can't compare which one is more vigorous yet. Many growing points have put out as many as 4~5 new open traps after they were potted. Also, as you can see in the picture, the traps are getting larger each time significantly. I don't know if Matt has applied different hormone ratios when they were in TC but it had so large root system compared to other varieties even when they were inside the tube as a tissue culture. Compared to B52 and A2, the vigrousness of DC XL seems to come from the power to grow large root system quickly. B52 seems to grow large upper parts regardless of the roots. When B52 was inside the tube, it had tiny roots compared to the upper green parts.
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DC XL 27th Jan
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DC XL 4th Feb
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I added this plant because I think it is not well known to you. It is called Red-Green. I bought it from Jang Gi-Won who also developed the Korean Melody Shark. It has relatively short petioles, thin and long red teeth and the traps are currently about an inch in size. It was the most vigorous plant before I received tissue cultures from FTS. For example, I had experiemented my plants by trying so many different kind of fertilizers, enzymes and hormones. This is the only cultivar that was not affected by so many repotting and so many chemicals. Other cultivars such as Fuzzy Tooth and South-West Giants do not even have a normal-shaped traps at the same condition. The margins of petioles and traps become black when they are polluted by chemicals and minerals but this Red-Green does not show significant loss.
By the way, all my plants are currently skipping dormancy.
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Red Green
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Last edited by luxiloid on Mon Feb 06, 2012 4:08 am, edited 3 times in total.
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Re: Flytraps acclimating from tissue culture

Postby Steve_D » Sat Feb 04, 2012 4:35 pm

Wow, Luxiloid, all the plants look great! :D

A2 (the sibling of B52) is a wonderful vigorous and large trapped plant, definitely a great companion for B52 in a collection or breeding program. The DC XL, which I have grown for 3 years from a single small plant, amazed my last year in Spring with its first flush of growth after dormancy, with huge, wide, glossy leaves and gigantic traps. I think that DC XL might compete favorably with B52 for largest traps, and all three plants (A2, B52 and DC XL) are great for giant-trapped Venus Flytrap enthusiasts.

Matt has been tissue culture propagating DC XL and he and I are both growing some of the DC XL tissue-culture babies. We hope to have a good number of young DC XL Flytraps to offer at FlytrapStore.com this year for American customers. We're also working hard on propagating and growing out A2 to offer as well.

In addition to those plants, I think that Low Giant and Dutch Delight deserve a place in anyone's giant-trapped Venus Flytrap collection, with perhaps Big Vigorous as a companion as well. :D

Anyway, it's great to see Matt's tissue-culture plants that spent their life in a vial or flask, planted out and growing so well in another country. Where are you located, Luxiloid? (Your location is not mentioned in the part of your profile that appears with your posts.)

Thanks so much for posting these great photos and comments. :)
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Re: Flytraps acclimating from tissue culture

Postby Matt » Sat Feb 04, 2012 7:42 pm

Wow luxiloid! Those plants look amazing! You've done very well with them and they're definitely on their way to being some really nice plants after getting established. Plants usually look their best about 3 to 4 months out of tissue culture, so you have a lot to look forward to because they're already looking really good :)
luxiloid wrote:I don't know if Matt has applied different hormone ratios when they were in TC but it had so large root system compared to other varieties even when they were inside the tube as a tissue culture.

All of the plants were growing on the same media since initialization in tissue culture and haven't been on any hormones since July or August. Some clones just grow much better in tissue culture than others and it doesn't necessarily directly translate to how they grow once in soil. For example, Wacky Traps grows extremely well in vitro, but is very challenging to grow to a large size in vivo. DC XL grows very well in vitro and in vivo. Sawtooth and most of the sawtooth-like flytraps tend to stay extremely small in vitro, but grow quite well in vivo. It's been interesting observing the difference in growth in vitro and in vivo for many different Venus fly trap clones over the last few years.
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Re: Flytraps acclimating from tissue culture

Postby jht-union » Sat Feb 04, 2012 9:42 pm

Wow, those look great! :)
Thanks for posting! :)
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Re: Flytraps acclimating from tissue culture

Postby swat007j » Sat Feb 04, 2012 10:01 pm

Amazing !
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Re: Flytraps acclimating from tissue culture

Postby luxiloid » Sun Feb 05, 2012 1:12 am

Steve_D wrote:Wow, Luxiloid, all the plants look great! :D

Thank you, Steve.

Steve_D wrote:A2 (the sibling of B52) is a wonderful vigorous and large trapped plant, definitely a great companion for B52 in a collection or breeding program. The DC XL, which I have grown for 3 years from a single small plant, amazed my last year in Spring with its first flush of growth after dormancy, with huge, wide, glossy leaves and gigantic traps. I think that DC XL might compete favorably with B52 for largest traps, and all three plants (A2, B52 and DC XL) are great for giant-trapped Venus Flytrap enthusiasts.

There is no B52 in the market here so I have been wanting this plant for so long. However, I don't know why but in my conditions ,the A2 and DC XL are doing better than B52 right now. I have to see for some more time but maybe it is because the B52 had so tiny roots when they were planted.

Steve_D wrote:In addition to those plants, I think that Low Giant and Dutch Delight deserve a place in anyone's giant-trapped Venus Flytrap collection, with perhaps Big Vigorous as a companion as well. :D

Really? I will order Dutch Delight right away :D

Steve_D wrote:Anyway, it's great to see Matt's tissue-culture plants that spent their life in a vial or flask, planted out and growing so well in another country. Where are you located, Luxiloid? (Your location is not mentioned in the part of your profile that appears with your posts.)

It is so wonderful that I can buy plants from over tha Pacific Ocean. The sterile state of the TC allows easier trade too. I leave in South Korea, by the way.

Steve_D wrote:Thanks so much for posting these great photos and comments. :)

Thanks you too.

Matt wrote:Wow luxiloid! Those plants look amazing! You've done very well with them and they're definitely on their way to being some really nice plants after getting established. Plants usually look their best about 3 to 4 months out of tissue culture, so you have a lot to look forward to because they're already looking really good

Thank you. It is all because of your nice TCs.

Matt wrote:All of the plants were growing on the same media since initialization in tissue culture and haven't been on any hormones since July or August. Some clones just grow much better in tissue culture than others and it doesn't necessarily directly translate to how they grow once in soil. For example, Wacky Traps grows extremely well in vitro, but is very challenging to grow to a large size in vivo. DC XL grows very well in vitro and in vivo. Sawtooth and most of the sawtooth-like flytraps tend to stay extremely small in vitro, but grow quite well in vivo. It's been interesting observing the difference in growth in vitro and in vivo for many different Venus fly trap clones over the last few years.

Thank you for the information. I grow the plants in coconut coir mix and I found that even among the flytraps, there seems to be certain level of ariation and pH levels for each of the cultrivars. Maybe, more complicated :roll:
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Re: Flytraps acclimating from tissue culture

Postby Stratofortress » Sun Feb 05, 2012 1:19 am

The new leaves are growing so red that I cannot compare it to Red Dragon (Akai Ryu). I was expecting some greener margin or so but till now they are so identical.


I have a number of small Green Dragon specimens myself, and I agree, the only apparent difference thus far seems to be exceptionally long petioles. All of my GDs are still young plants, so I'll probably have to wait few years to see if they grow into the size and color characteristics typical of the cultivar

Those are all Astonishing VFTs. I'm always very entertained to see new big bright photos posted in the forum. Much Thanks :D
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Re: Flytraps acclimating from tissue culture

Postby Stratofortress » Sun Feb 05, 2012 1:32 am

Steve_D wrote:Matt has been tissue culture propagating DC XL and he and I are both growing some of the DC XL tissue-culture babies.


I can't remember if it was Steve or Matt, but either or posted some images of the largest flytraps I've ever seen in photos anywhere. I have thousands and thousands of Dionaea images alone, but nothing compares to the plants below.
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What is the origin and destiny of these beasts?
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Re: Flytraps acclimating from tissue culture

Postby Darkrai283 » Sun Feb 05, 2012 11:08 am

OMG! Those are HUGE!!! The one on the right has some abnormal rhizome! :? It looks like a sarr! :lol:
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Re: Flytraps acclimating from tissue culture

Postby Steve_D » Sun Feb 05, 2012 3:03 pm

Darkrai283 wrote:The one on the right has some abnormal rhizome!

That often happens in the Spring just after a plant produces a flowerstalk; a large mass of tissue forms from which new leaves emerge. :)
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Re: Flytraps acclimating from tissue culture

Postby Matt » Sun Feb 05, 2012 5:07 pm

Stratofortress wrote:I can't remember if it was Steve or Matt, but either or posted some images of the largest flytraps I've ever seen in photos anywhere. I have thousands and thousands of Dionaea images alone, but nothing compares to the plants below.
Image
What is the origin and destiny of these beasts?

I posted those photos in this thread:
dc-xl-if-you-love-big-traps-be-sure-to-look-t11164.html

The origin of the plant for me was from David Conner via Steve who received a small plant as a gift from David a few years ago. I've contacted David Conner to try to track down the original grower of this monster, but he doesn't recall who he got the plant from originally.

DC XL has produced larger traps for me than any of my B52s ever have. In the photos in the above linked thread, those are the first traps of the season being produced as the plants exit dormancy. I snipped the flower stalks off of them to use for tissue culture, so that allowed them to immediately produce some very large traps.
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Re: Flytraps acclimating from tissue culture

Postby Stratofortress » Sun Feb 05, 2012 6:08 pm

I posted those photos in this thread:
dc-xl-if-you-love-big-traps-be-sure-to-look-t11164.html

The origin of the plant for me was from David Conner via Steve who received a small plant as a gift from David a few years ago. I've contacted David Conner to try to track down the original grower of this monster, but he doesn't recall who he got the plant from originally.

DC XL has produced larger traps for me than any of my B52s ever have. In the photos in the above linked thread, those are the first traps of the season being produced as the plants exit dormancy. I snipped the flower stalks off of them to use for tissue culture, so that allowed them to immediately produce some very large traps.


Could DC XL be the same cultivar as "Giant Form", or "B52 x Giant Form"? I suppose it's impossible to be certain.
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Re: Flytraps acclimating from tissue culture

Postby Matt » Sun Feb 05, 2012 6:33 pm

Stratofortress wrote:Could DC XL be the same cultivar as "Giant Form", or "B52 x Giant Form"? I suppose it's impossible to be certain.

I don't think so, but it could be possible.
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Re: Flytraps acclimating from tissue culture

Postby luxiloid » Mon Feb 06, 2012 1:34 am

Stratofortress wrote:Could DC XL be the same cultivar as "Giant Form", or "B52 x Giant Form"? I suppose it's impossible to be certain.

The DC XL looks very much like the Giant Form. They are both erect and both green plants with little bit red inside the trap. However, looking at my little DC XL just out of TC and Matt's mature picture, I can see that the petioles and traps of the DC XL is trying to be more erect than the Giant Form. The shape of the trap is more sharp and crowded with hairs at both ends for the DC XL than the roundness of the Giant Form.
edge_comp2.jpg
Comparing the traps
edge_comp2.jpg (330.14 KiB) Viewed 2502 times


Comparing the DC XL and B52 is much easier. They are very different for the petioles, traps and the root system. Looking at the thick and massive roots of the DC XL, I even feel as if they are different species. My little DC XL just came out of the TC has grown so much roots that some came out of the bottom of my pots already in just four weeks. For the same size of the plant, DC XL seems to grow very thick roots with massive amounts of hairs.

I think it would be very interesting to cross this DC XL to already famous giants like B52. With strong root from DC XL and vigrous upper growth of B52, the seedling may easily exceed the limit of two inches barrier.
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Re: Flytraps acclimating from tissue culture

Postby luxiloid » Mon Feb 06, 2012 1:40 am

Stratofortress wrote:
The new leaves are growing so red that I cannot compare it to Red Dragon (Akai Ryu). I was expecting some greener margin or so but till now they are so identical.


I have a number of small Green Dragon specimens myself, and I agree, the only apparent difference thus far seems to be exceptionally long petioles. All of my GDs are still young plants, so I'll probably have to wait few years to see if they grow into the size and color characteristics typical of the cultivar

Those are all Astonishing VFTs. I'm always very entertained to see new big bright photos posted in the forum. Much Thanks :D


I read that the GD is a tissue culture mutation of the RD but it has larger traps. The fact that it is larger and grows faster will make the edges look more greener. I can't wait to see it getting matured.
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